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Presented and discussed the new data of methane quantity, isotopic values 13C and D in methane of two types of intrasedimental ground ice, West Yamal, Russia. The first type of intrasedimental ground ice is icy sediments (IS) not more than 2-3 m thick. The second type of intrasedimental ground ice is large massive tabular ice bodies more than 20 m thick. The extraction of gases accomplished following the dry extraction technique. One approach that has been used with success to determine the origin of methane is the analysis of the δ13C and D value of CH4 gases. Our data of isotopic measurements of the 13C -70,5pm, and D -326pm in methane are typical for gas formed with the participation of vital functions of bacteria.