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The tasks of this work include the grid square mapping of the plants that are under danger of extinc-tion; geographical analysis of biodiversity distribution; development of effective ecological conservation strategies for specific regions. The study carried out in two regions of Russia: Kaliningrad oblast’ as an ex-ample of urbanized European north-west and Crimean peninsula. The species of higher plants listed in the Red Books have been considered as the basic units of biodiversity. The results show the possibility of using the geo-botanical monitoring records to identify priority areas for biodiversity conservation.