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In this study we propose a new approach to monitoring of the removal of luminescent nanocomposites and their components with urine using artificial neural networks. A complex multiparametric problem of optical imaging of synthesized nanocomposites - nanometer graphene oxides, covered by the poly(ethylene imine)–poly(ethylene glycol) copolymer and by the folic acid in a biomaterial is solved. The proposed method is applicable for optical imaging of any fluorescent nanoparticles used as imaging nanoagents in biological tissue.