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The lack of donor organs is a major problem in modern medicine. A promising solution to this problem is the fabrication of human tissues and organs using three-dimensional bioprinting. Most existing biofabrication methods use scaffolds made from biomaterials or nanomaterials. A recently proposed magnetic levitation method for the first time used an approach without scaffolds and labels; however, the complex spatial structures of the assembled tissue structures were not available yet [1]. Acoustic biofabrication of 3D tissue constructs represents a novel rapidly developing scaffold-free and label-free approach, allowing to assemble complex tubular structures. This technology can be used in the constructing of blood vessels, an essential part of any organ.