ИСТИНА

Tissue engineering is a fast growing area of regenerative medicine aiming at structural and functional regeneration or replacement of damaged tissues and organs. Human mesenchymal stem/stromal cells (MSC) combined with different kinds of scaffolds are often used for tissue engineering. MSC produce many bioactive factors involved in multiple reparative and regenerative processes in damaged tissues. MSC conditioned medium (MSC-CM), contained a combination of various secreted components produced by MSC, could mediate most of beneficial regenerative effects of MSC and lack possible side-effects of using MSC themselves. We have developed a prototype of a novel biomaterial for regenerative medicine based on the combination of MSC-CM with different types of collagen scaffolds. We have suggested the optimized protocol of human adipose-derived MSC-CM production based on the dynamics of key growth factors secreted by cells during long-term conditioning in clinically compliant media. MSC-CM demonstrated specific activity in vitro by stimulating the migration of human fibroblasts and endothelial cells, which are both important for damaged tissue reparation and regeneration. We have combined MSC-CM both with collagen gel to produce a biomaterial for injection and with collagen wound dressing. We have revealed an optimal collagen/CM relation to manufacture scaffolds with appropriate mechanical structure and MSC secreted factors release. The regenerative effects of the manufactured constructs were successfully tested in vitro and in vivo for wound healing and spermatogenesis recovery. The developed biomaterials could be further improved to enhance their therapeutic activity and produce new modes for various clinical applications.