ИСТИНА

In 2012 the publication “ENCODE Project Writes Eulogy For Junk DNA” demonstrated that 76% of the genome is transcribed and is functionally active. It gave a boost to many studies on the functions and molecular mechanisms of non-coding RNAs. It turns out that non-coding RNAs may participate in transcriptional regulation processes, chromatin remodeling, and chromatin architecture maintenance. For the present various techniques have been developed to localize specific RNAs on chromatin. These include Chromatin Isolation by RNA Purification (ChIRP), Capture Hybridization Analysis of RNA Targets (CHART), RNA Affinity Purification (RAP-DNA), and others. In 2017, works began to appear that offered methods to obtain genome-wide data on all potential RNA-chromatin interactions in a cell: MARGI, RED-C, and GRID-seq. We developed a database using a column-oriented database management system ClickHouse and populated it with equally preprocessed data. Moreover, we designed a user-friendly web-interface (http://rnachrom.bioinf.fbb.msu.ru/), that allows the user to: send the RNA-chromatin contacts profile in Genome Browser and compare it with ENCODE datasets (for example with epigenomic tracks); visualize analytic plots/tables of specific RNA–/group of RNAs – chromatin interactions; show a list of RNAs with different filters that contains a meta-information and normalized values of their contactability; compare experiments; download the raw/normalized data in order to make user’s own normalization, filtering, or any other analysis; FUNDING: this work was supported by RFBR grant #20-04-00459 А