ИСТИНА

Background & objectives. Generally accepted scales for assessing immune microenvironment in gastric cancer are based on manual calculation of average number of cells in hot spots (predominantly intratumoral and in invasive margin). This is time-consuming process, development of new assessment scales is required. Methods. After immunohistochemical staining average area of CD8+ cells (square micrometers) in three fields of view (lens magn.x20) was assessed separately in central part of tumor and in morphologically normal mucous membrane of peritumoral region directly adjacent to tumor tissue. Automatic segmentation method based on color separation (RGB system) implemented in the Leica LASX tool was used. Results. We also counted average number of cells in three hot spots (lens magn.x20) in tumor and normal mucous membrane. Multivariate analysis of factors affecting overall survival was performed using Cox proportional hazard model (N=43). Risk ratio (RR) for intratumoral CD8+ cells using hot spot counts was 1,001 (CI: 0,995–1,008, p<0,01) and when using the new scale RR=1,009 (CI: 0,532–1,914, p<0,01). By using the hot spot count obtained RR for CD8+ cells in normal mucosa using hot spot calculation was 1,005 (CI: 0,999–1,011, p<0,01) and when using the new scale RR=1,599 (CI: 0,519–4,917, p<0,01). Conclusion. Our proposed scale for assessing immune microenvironment in gastric cancer showed that average area of CD8+cells assessed in normal mucosa by automatic segmentation is strong negative prognostic factor. Values obtained using hot spot calculation were not significantly related to prognosis. Ссылка на абстракт: https://www.esp-congress.org/scientific-programme/online-programme.html#!/details/abstract/1246 Ссылка на программу конгресса: https://www.esp-congress.org/scientific-programme/online-programme.html#!/by-topic/6