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The main factors determining the permeation properties of the GO membranes are the state and mobility of sorbed liquids within the interplane space of swollen graphite oxide. The influence of the orientational ordering of the graphene planes on membrane permeability is also very likely. Experimental data on the mechanism of transmembrane diffusion are limited. Electron paramagnetic resonance spectroscopy (EPR) is widely used for the study of the structure and dynamics of various materials – polymers, glasses, liquid crystals, biological tissues, etc. The present report is devoted to the potential of the spin probe technique for the investigation of graphite oxide materials.