ИСТИНА

Antimicrobial activity of silver nanoparticles (Ag-NP) against antibiotic-resistant bacteria strains led to an active application of Ag-NP in medical practice and festering wound treatment. The long-lasting wounds such as bedsores, burns, purulent wounds are an enabling environment for pathogenic flora. To increase the rate of wound healing of such wounds various Zn compounds are currently used. In this regard, a combination of Ag-NP and Zn in one treating complex could increase the productivity of wound healing and reduce the possibility of its infection. However, in addition to antibiotic activity, Ag-NP may show the cytotoxic effect due to an elevation of oxidative stress in human cells. To avoid this negative effect the utilization of humic substances (HS) both as a reduction agent for the formation of Ag-NP and an antioxidant to reduce the cytotoxicity of synthesized Ag-NPs is a promising technique. Besides the ability to reduce Ag HS is also a natural complexing ligand that could form stable humate complexes with metal ions including Zn. For this reason, the aim of the present study was to optimize the synthesis of the combined preparation of Ag-NP/Zn humate as a promising agent for wound healing with intensive antimicrobial activity.