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Increasing the number of antibiotic-resistant bacteria requires the development of biocompatible antibacterial materials. In this report, we studied the effect of Pt, Fe nanoparticles and their combinations distributed on the surface of the TiCaPCON bioactive coating on the antibacterial properties of the material, taking into account factors contributing to the death of bacteria: the release of bactericidal ions into the biological environment, the formation of reactive oxygen forms and the formation of microgalvanic pairs between the nanoparticles and the surrounding matrix. Coating TiCaPCON was precipitated on a silicon wafer by magnetron sputtering of composite SHS-target TiC–CaO–Ti3PO (x). PT, Fe particles and their combinations were obtained by implantation of metal ions into the TiCaPCON coating. The formation of metal nanoparticles on the surface was due to their limited solubility in the crystal lattice of the coating. To change the size and distribution of nanoparticles on the surface, some samples were annealed at a temperature of 700 C for 1 h. Thus, samples with particles: Pt, Fe, Ptan, Fe,(Pt+ Fe), Ptan+ Fe (Pt+ Fe) were obtained and investigated.