ИСТИНА

Blood plays crucial role in delivering oxygen and other substances to body tissues. Red blood cells (RBC) as blood component are the most abundant cells in the blood. RBCs interact with each other resulting in formation of RBCs linear or 3D aggregates. The process of RBC aggregates formation is referred to as RBC aggregation and it mainly determines the blood viscosity. RBC spontaneous aggregation and shear forced RBC disaggregation depend on a variety of medium and cell factors. Blood coagulation mainly depends on platelet aggregation. Platelet aggregation also depends on a variety of factors and determines hemostasis. Deviation of RBC and platelets aggregation parameters from the healthy conditions can significantly impair blood microcirculation in the body, as well as lead to the development of vascular pathologies and comorbidities. Elevated RBC and platelet aggregation were be found in the blood of patients suffering from type 2 diabetes mellitus, cardiovascular diseases, cerebrovascular disorders, and other pathologies [A. Maslianitsyna, et al. Diagnostics (Basel) 11(1):76 (2021)]. The aim of this study is to show the comprehensive microhemorheological and microcirculatory patterns of hypertensive patients obtained by modern and simple optical techniques. In this study, light scattering techniques were used to measure RBC and platelet aggregation properties in vitro. These methods are based on the dependence of scattered light intensity on particle size and allow for the registration of RBC and platelet aggregation kinetics. RheoScan-AnD300 device (RheoMedTekh, Republic of Korea) was used to measure several RBC aggregation parameters. Biola-230LA (Biola, Russia) was used to determine the platelet aggregation under adenosine diphosphate activation. Digital capillaroscope Kapillaroskan-1 (AET, Russia) was used to visualize the terminal blood capillaries in the nail bed of patients in vivo and to calculate blood flow velocities therein. Comprehensive study of 50 patients confirms the hypothesis of increased RBC and platelet aggregation in the blood samples of patients suffering from arterial hypertension compared to that of healthy donors. Our results also clearly demonstrate the possibility of using light scattering techniques to assess changes in microrheological (RBC aggregation and platelet aggregation) and, therefore, microcirculatory parameters (capillary blood flow velocity etc.). In future, changes in RBC and platelets aggregation parameters measured in vitro can be used to correct and develop the therapy protocols for patient suffering from hypertension. The study was supported by the Russian Science Foundation Grant No. 22-15-00120.