ИСТИНА

Detection of microorganisms is of great demand for various producing processes in biotechnology, medicine, industry, etc. Detection of microorganisms with various sensors requires preconcentration of microorganisms on sensor surface in order to achieve the desired detection limits. Preconcentration can be provided via: charging surfaces (polylysine, polychitosan), biorecognition elements (antibodies, aptamers, lectins etc.) and biomimetics of bioaffinity interactions. The latter is of particular interest due to combination of selectivity and operational stability. Phenylboronic acids are widely used for detection of cell wall components – sugars and hydroxy acids. However, majority of existing conductometric and impedimetric systems generate both specific and non-specific signals as the increase of resistance and therefore they are doubtful about practical importance. In this work we demonstrate novel sensing material – conducting boronate-substituted polyaniline [1]. Sensing principle is reagentless and is based on resistance decrease in the presence of microorganisms that allows discriminating between specific and non-specific interactions since the latter always results in resistance increase. Developed sensing material was successfully applied for Penicillium chrysogenum detection. Polymer-modified interdigitated microelectrodes demonstrated the increase of conductivity in the presence of the mold. 1. E.A. Andreyev, M.A. Komkova, V.N. Nikitina, N.V. Zaryanov, O.G. Voronin, E.E. Karyakina, A.K. Yatsimirsky, A.A. Karyakin // Analytical Chemistry. – 2014. – V. 86. – № 23. – P. 11690–11695 Russian Science Foundation financial support (grant no. 16-13-00010) is greatly acknowledged.