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Today, magnetic nanoparticles (MNPs) are widely used in biomedicine, catalysis, data storage, environmental remediation and sensorics. Cobalt oxides are of interest due to their relatively high magnetic moment, spinel structure, unique properties and low cost [1]. Pulsed laser ablation in liquid (PLAL) is a universal, efficient and "green" method for producing chemically pure cobalt oxide MNPs in a one-step process, without the use of chemical reagents. The advantages of the PLAL method are the ability to control the size and composition of MNPs by varying the laser radiation parameters and the choice of buffer liquid. Using PLAL targets of thin films of varying thicknesses instead of a bulk target for MNPs production potentially adds an additional possibility to control MNPs sizes. In our work, we studied PLAL generation of colloidal MNPs in distilled water from thin (5–500 nm) Co films magnetron sputtered. These films were irradiated by a Nd:YAG laser EKSPLA PL 2143A (1064 nm, 34 ps).