ИСТИНА

The subfamily Papilionoideae of Leguminosae is characterized by numerous rearrangements of the plastid genome, especially in the IRLC (Inverted Repeat Lacking Clade). The tribe Loteae includes 16 genera and about 280 species of herbs, semi-shrubs and shrubs, common in Eurasia, Africa, Australia, Oceania, North and South America. In the phylogenetic tree of legumes, the tribe Loteae is located close to the IRLC. The genera Robinia and Sesbania, phylogenetically close to Loteae, showed the presence of large rearrangements in the LSC (large single copy) region of the plastid genome: 36 kb inversion in Robinia and 50 kb reversion in Sesbania. The slow evolution of plastid sequences has led to a lack of proper resolution in the relationship between the large evolutionary branches within Loteae by plastid markers, which can be overcome by whole genome sequencing. The main objectives of the study include: 1. Complete reading and annotation of ca.15 plastid genomes of representatives of large genera of the tribe Loteae. 2. Conducting structural and phylogenetic analyses of the plastomes. All plastomes were sequenced using Illumina platform. To date, we have 22 plastid genomes (including six from GenBank), which belong to 20 species from six genera of the tribe Loteae (Acmispon, Anthyllis, Coronilla, Hippocrepis, Hosackia and Lotus), as well as Sesbania cannabina and Robinia pseudoacacia as ougroups. The results obtained and preliminary conclusions: 1. The plastomes of representatives of the tribe Loteae have a conservative structure with only minor rearrangements, unlike those of the closest related groups (Robinia and Sesbania). 2. The genome-wide plastid data showed a good phylogenetic signal, which allowed to resolve the relationship between the large evolutionary branches of the tribe, such as Hippocrepis, Coronilla and Anthyllis. The study is supported by the Russian Science Foundation, project 23-24-00052. Key words: Loteae, phylogeny, plastid genome, rearrangements.