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We report here immobilization of both glucose oxidase and lactate oxidase into N-substituted polypyrrole films on Prussian Blue modified electrodes. The enzymes have been immobilized onto screen-printed electrodes modified with Prussian Blue produced by LTD RUSENS (Russia). The biosensors have been tested in FIA regime. The analytical characteristics of resulted biosensors (response time, sensitivity, dynamic range, detection limit, operational stability, storage stability, reproducibility) are similar to those observed for the corresponding biosensors made without electropolymerization.