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Dried-Reswollen Immobilized Biocatalysts for Detoxification of Organophosphorous Compounds in the Flow Systemsстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Efremenko E.N., Lyagin I.V., Plieva F.M., Galaev I.Y., Mattiasson B.
- Журнал: Applied Biochemistry and Biotechnology
- Том: 159
- Год издания: 2009
- Издательство: Humana Press, Inc.
- Местоположение издательства: United States
- Первая страница: 251
- Последняя страница: 260
- DOI: 10.1007/s12010-009-8564-5
- Аннотация: New immobilized biocatalysts based on polypeptides containing N- or C-terminal polyhistidine sequences and possessing organophosphorus hydrolase activity were investigated for detoxification of organophosphorous neurotoxic compounds in the flow systems. The biocatalysts were revealed to have a high catalytic activity within wide pH and temperature ranges 7.5–12.5 °C and 15–65 °C, respectively. The immobilized biocatalysts can be dried and reswollen before use with 92–93% catalytic activity remaining after drying and rehydration procedures. The half-lives of the biocatalysts under wet and dry storage conditions were 420 and 540 days, respectively.
- Добавил в систему: Лягин Илья Владимирович