Fiber-Optic Immunosensor for Detection of Crimean-Congo Hemorrhagic Fever IgG Antibodies in Patientsстатья
Статья опубликована в высокорейтинговом журнале
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Дата последнего поиска статьи во внешних источниках: 19 сентября 2015 г.
Аннотация:Crimean-Congo hemorrhagic fever (CCHF) is a severe viral disease with high fatality rate.
CCHF virus is endemic in parts of Africa, Asia, Middle East and southeastern Europe.
Rapid diagnostics of CCHF is vital for appropriate clinical management and prevention of
secondary spread from human-to-human. Currently, diagnostics relies on Real-Time RTPCR
and antibody or antigen detection using ELISA. These methods require trained
personnel and expensive equipment and are not appropriate for point-of-care (POC)
diagnostics. Furthermore there are no POC assays available for CCHF.
We developed a fiber-optic biosensor for the detection of CCHF IgG antibodies. In order
to improve sensitivity, we optimized both the bioreceptor immobilization protocol and
the chemiluminescence substrate formulation. The resulting protocol showed a 100-fold
greater sensitivity for detection of CCHF antibodies. Finally, we evaluated the fiber-optic
biosensor with two CCHF patient sera. We showed that the fiber-optic biosensor is 10-
times more sensitive that colorimetric ELISA and is able to detect both patients with
high and low levels of IgG antibodies. We believe that the fiber-optic biosensor is a
suitable alternative to ELISA as it is much more sensitive and makes it possible to detect
small amount of antibodies at an early stage of infection, and can be integrated as a
point-of-care diagnostic system of CCHF.