Development of a chemiluminescent enzyme immunoassay for the determination of dexamethasone in milkстатья
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:An indirect competitive chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) for the
determination of dexamethasone (DEX) was developed using soybean peroxidase (SbP) as an enzyme
label. A mixture of 3-(100-phenothiazinyl)-propane-1-sulfonate (SPTZ) and 4-morpholinopyridine
(MORPH) was used as an enhancer of SbP-induced chemiluminescence. Varying the concentrations of
the capture antigen (DEX-ovalbumin) and specific anti-DEX antibody, the conditions of the assay
were optimized. The values of IC10, IC50 and working range (IC20–IC80) of the CL-ELISA of DEX were
0.02, 0.9, 0.08–9.3 ng mL1, respectively. It was shown that a pretreatment of cow milk samples by
centrifugation and 25% methanol prevented the matrix effect of whole milk. The coefficient of variation
(CV) and recovery values from the spiked milk samples estimated by the developed CL-ELISA were in
the range of 2.2 to 9.9% and 82 to 142%, respectively.