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Levels of major selenoproteins in T cells decrease during HIV infection and low molecular mass selenium compounds increaseстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 10 августа 2018 г.
- Авторы: Gladyshev V.N., Stadtman T.C., Hatfield D.L., Jeang K.T.
- Журнал: Proceedings of the National Academy of Sciences of the United States of America
- Том: 96
- Номер: 3
- Год издания: 1999
- Издательство: National Academy of Sciences
- Местоположение издательства: United States
- Первая страница: 835
- Последняя страница: 839
- DOI: 10.1073/pnas.96.3.835
- Аннотация: It has been observed previously that plasma selenium and glutathione levels are subnormal in HIV-infected individuals, and plasma glutathione peroxidase activity is decreased. Under these conditions the survival rate of AIDS patients is reduced significantly. In the present study, using 75Se-labeled human Jurkat T cells, we show that the levels of four 75Se-containing proteins are lower in HIV-infected cell populations than in uninfected cells. These major selenoproteins migrated as 57-, 26-, 21-, and 15-kDa species on SDS/PAGE gels. In our earlier studies, the 57-kDa protein was purified from T cells and identified as a subunit of thioredoxin reductase. The 26- and 21-kDa proteins were identified in immunoblot assays as the glutathione peroxidase (cGPX or GPX1) subunit and phospholipid hydroperoxide glutathione peroxidase (PHGPX or GPX4), respectively. We recently purified the 15-kDa protein and characterized it as a selenoprotein of unknown function. In contrast to selenoproteins, low molecular mass [75Se]compounds accumulated during HIV infection and migrated as a diffuse band near the front of SDS/PAGE gels.
- Добавил в систему: Гладышев Вадим Николаевич