Protein engineering. New paradigm for creation of biocatalysts

Tishkov, V.I.; Alekseeva, A.A.; Zarubina, S.A.; Dolina, I.A.; Atroshenko, D.L.; Stepashkina, A.V.; Poloznikov, A.A.; Zakharova, G.S.; Chubar, T.A.; Gazaryan, I.G.; Fedorchuk, V.V.; Sklyarenko, A.V.; Yarotsky, S.V.; Savin, S.S.

Авторы: Tishkov V.I., Alekseeva A.A., Zarubina S.A., Dolina I.A., Atroshenko D.L., Stepashkina A.V., Poloznikov A.A., Zakharova G.S., Chubar T.A., Gazaryan I.G., Fedorchuk V.V., Sklyarenko A.V., Yarotsky S.V., Savin S.S.
Сборник: Program and Abstract Book of International Conference "Biocatalysis-2015: Fundamentals and Applications", Moscow region, Avantel Club Istra
Тезисы
Год издания: 2015
Место издания: Innovations and High Technologies MSU Ltd Moscow
Первая страница: 31
Последняя страница: 31
Аннотация: Enzymes are unique biocatalysts whish provide very high substrate-, region- and stereo-selectivity. Processes catalyzed by enzymes are characterized by high yield of target product (up to 100%), very low amount or absence of side products and mild conditions of reaction. High specificity of enzyme at the same time is significant drawback because during evolution biocatalysts were opti-mized to natural product. Even small change of structure of natural compound could result in very high decrease or absence of activity. The second important fact is that pH-optimum activity differs from optimal process conditions. Other drawback of many proteins is insufficient stability. That is why, previously, real conditions of biotechnology processes were compromise between enzyme properties and best reaction conditions. Development of protein engineering methods resulted in total change of biotechnology processes construction. At first, optimal reaction conditions are selected and in the sec-ond stage bioinformatic methods are used to select initial enzyme, which the engineered by recombi-nant DNA technologies. The other way is to look for and use enzymes with new catalytic activity for production of well known products. In this report we will present use of this approaches to create biocatalysts with desired and new properties for formate dehydrogenases, D-amino acid oxidase, peroxidase, penicillin acylase, hy-drolase of alpha-amino acid esters etc. The work was supported by Russian Foundation for Basic Research (RFBR), grants 13-04-01907-а, 13-04-01909-а, 13-04-02013-а, 13-04-40207-H KOMFI, 14-04-32042-mol_a, 14-04-00865-a, 14-04-01312-a, 14-04-01625-a, 14-04-01665-a, 15-54-78035-Ital_a.
Добавил в систему: Тишков Владимир Иванович

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