Formate dehydrogenases from eukaryotes

Alekseeva, A.A.; Dolina, I.A.; Kargov, I.S.; Savin, S.S.; Tishkov, V.I.

Авторы: Alekseeva A.A., Dolina I.A., Kargov I.S., Savin S.S., Tishkov V.I.
Сборник: Program and Abstract Book of International Conference "Biocatalysis-2015: Fundamentals and Applications", Moscow region, Avantel Club Istra
Тезисы
Год издания: 2015
Место издания: Innovations and High Technologies MSU Ltd Moscow
Первая страница: 43
Последняя страница: 43
Аннотация: Formate dehydrogenase (FDH, EC 1.2.1.2.) is an enzyme which is widely occurred in differ-ent prokaryotes and eukaryotes. FDH is also used in biotechnology. It catalyses the reaction of formate oxidation to carbon dioxide coupled with reduction of NAD(P)+ to NAD(P)H. Due to the irreversibil-ity of catalyzed reaction, low price of formate ion and wide pH optimum of activity, formate dehydro-genase is an excellent biocatalyst for cofactor regeneration. New technologies of recombinant protein production allow us to obtain enzymes from differ-ent sources. Genes, encoded FDH from different bacteria, yeasts and even plants were successfully cloned and expressed in our laboratory. Now we've focused on eukaryotic genes due to the fact that FDHs from eukaryotes may have better kinetic properties then bacterial enzymes. Several years ago two genes of plant FDH were cloned – from the model plant Arabidopsis thaliana (AthFDH) and soybean Glycine maxi (SoyFDH). It was shown that SoyFDH has the lowest values of Michaelis constants among all studied FDHs but is not thermally stable compared to bacte-rial formate dehydrogenases. More than 10 mutant forms of SoyFDH were obtained by genetic engi-neering method and some of them are as stable as bacterial enzymes, but have kinetic properties as plant FDHs. Now two more eukaryotic genes were cloned – from moss Psychomitrella patens (PpaFDH) and yeast Ogataea parapolymorpha (OpaFDH). In the present work we provide the com-parison of properties of new formate dehydrogenases from different eukaryotes This work was supported by grant of Russian President MK-2304.2014.4 and Russian Foun-dation for Basic Research (grant RFBR 14-04-01665-a and14-04-01662-a).
Добавил в систему: Тишков Владимир Иванович

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