A novel strategy for isolation and determination of sugars and sugar alcohols from conifers

Sarvin, B.A.; Seregin, A.P.; Shpigun, O.A.; Rodin, I.A.; Stavrianidi, A.N.

Авторы: Sarvin B.A., Seregin A.P., Shpigun O.A., Rodin I.A., Stavrianidi A.N.
Журнал: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Том: 1092
Год издания: 2018
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Первая страница: 138
Последняя страница: 144
DOI: 10.1016/j.jchromb.2018.06.005
Аннотация: The ultrasound-assisted extraction method for isolation of 17 sugars and sugar alcohols from conifers with a subsequent hydrophilic interaction liquid chromatography-tandem mass spectrometry method for their determination is proposed. The optimization of extraction parameters was carried out using Taguchi – L9 (34) orthogonal array experimental design for the following parameters—a methanol concentration in the extraction solution, an extraction time, a type of plant sample and an extraction temperature. The optimal ultrasound-assisted extraction conditions were—MeOH concentration – 30% (water – 70%), extraction time – 30 min, type of plant sample – II (grinded leaves 2–4 mm long), extraction temperature – 60 °C. Pure water and acetonitrile were used as eluents in gradient elution mode to separate the analytes. Direct determination of multiple sugars and sugar alcohols was carried out using a mass spectrometric detector operated in a multiple reaction monitoring mode, providing detection limits in the range between 0.1 and 20 ng/mL and good analytical characteristics of the method without derivatization. The developed approach was validated by multiple successive extraction method applied to test its performance on a series of 10 samples, i.e. 2 samples per each of 5 genera: Abies, Larix, Picea, Pinus (Pinaceae) and Juniperus (Cupressaceae), widely distributed in the boreal conifer forests of Eurasia. The novel strategy can be used for profiling of sugars and sugar alcohols in a wide range of plant species.
Добавил в систему: Родин Игорь Александрович

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