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Cardiomyocytes derived from human embryonic stem cells in pro-survival factors enhance function of infarcted rat heartsстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 10 августа 2018 г.
- Авторы: Laflamme Michael A., Chen Kent Y., Naumova Anna V., Muskheli Veronica, Fugate James A., Dupras Sarah K., Reinecke Hans, Chunhui Xu, Hassanipour Mohammad, Police Shailaja, O'Sullivan Chris, Collins Lila, Chen Yinhong, Minami Elina, Gill Edward A., Ueno Shuichi, Yuan Chun, Gold Joseph, Murry Charles E.
- Журнал: Nature Biotechnology
- Том: 25
- Номер: 9
- Год издания: 2007
- Издательство: Nature Publishing Group
- Местоположение издательства: United Kingdom
- Первая страница: 1015
- Последняя страница: 1024
- DOI: 10.1038/nbt1327
- Аннотация: Cardiomyocytes derived from human embryonic stem (hES) cells potentially offer large numbers of cells to facilitate repair of the infarcted heart. However, this approach has been limited by inefficient differentiation of hES cells into cardiomyocytes, insufficient purity of cardiomyocyte preparations and poor survival of hES cell-derived myocytes after transplantation. Seeking to overcome these challenges, we generated highly purified human cardiomyocytes using a readily scalable system for directed differentiation that relies on activin A and BMP4. We then identified a cocktail of pro-survival factors that limits cardiomyocyte death after transplantation. These techniques enabled consistent formation of myocardial grafts in the infarcted rat heart. The engrafted human myocardium attenuated ventricular dilation and preserved regional and global contractile function after myocardial infarction compared with controls receiving noncardiac hES cell derivatives or vehicle. The ability of hES cell-derived cardiomyocytes to partially remuscularize myocardial infarcts and attenuate heart failure encourages their study under conditions that closely match human disease.
- Добавил в систему: Наумова Анна Владимировна