|
ИСТИНА |
Войти в систему Регистрация |
ФНКЦ РР |
||
New multi-point mutants of D-amino acid oxidase from yeast Trigonopsis variabilisтезисы доклада
- Авторы: Zarubina S.A., Komarova N.V., Tishkov V.I.
- Сборник: Program and Abstract Book of International Conference "Biocatalysis-2015: Fundamentals and Applications", Moscow region, Avantel Club Istra
- Тезисы
- Год издания: 2015
- Место издания: Innovations and High Technologies MSU Ltd Moscow
- Первая страница: 52
- Последняя страница: 52
- Аннотация: D-amino acid oxidase (DAAO, EC 1.4.3.3) is a FAD-containing enzyme which fulfills physio-logical functions in living organisms. Also DAAO is the important biotechnological enzyme being used in biosensors and fine organic synthesis, especially in two step enzymatic conversion of cephalosporin C (CephC) into 7-amino cephalosporanic acid. In our laboratory gene of DAAO from yeast Trigonopsis variabilis (TvDAAO) was cloned and expressed in E.coli. Amino acid changes in positions 54 and 108 resulted in mutant enzymes with improved catalytic efficiency with CephC. Some mutations in cofactor-binding domain increased ther-mal stability catalytic efficiency with many D-amino acids. Here we present the results of combining these amino acid changes in multi-point mutants. The plasmids with mutations in tvdaao gene providing triple and quaternary amino acid substitutions were obtained. Mutant enzymes were expressed in E.coli cells, purified and characterized. It was found that new multi-point mutants of TvDAAO showed further enhancing in catalytic activity and thermal stabil-ity. Chromatographic activity determination with CephC was performed. The work was supported by Russian Foundation for Basic Research (grants 14-04-00865-a, 14-04-32064-mol_a)
- Добавил в систему: Тишков Владимир Иванович