|
ИСТИНА |
Войти в систему Регистрация |
ФНКЦ РР |
||
Composition of shoots endophytic flora of Syringa vulgaris plants from different populationsстатья
- Авторы: Krinitsina A.A., Churikova O.A., Konorov E.A., Speranskaya A.S.
- Сборник: Материалы международной научной конференции PLAMIC2018 «Растения и микроорганизмы: биотехнология будущего», 13-17 июня 2018 г
- Год издания: 2018
- Место издания: Уфа
- Первая страница: 42
- Последняя страница: 42
- Аннотация: The vital activity of endophytic bacteria, associated with plants, doesn`t affect the appearance and state of plants as a rule. The composition of endophytic communities which form in different organs (leaves, roots or shoots) may differ considerably as within one organism both in different individuals. Host genotype, plant age, soil type and geographical location as well may be involved in the composition and changeability of the shoot associated endophytic community. The composition of endophytic flora of buds and adjoined parts of shoots of Syringa vulgaris plants from Moscow (municipal green plantations and Botanical garden of Lomonosov Moscow State University), Moscow region (municipal green plantations) and Voronezh (green plantations near small office buildings) was analyzed. Plant material was collected during the physiological plant dormancy – in winter and early spring. After superficial sterilization the bud scales were removed in sterile conditions from buds and bark – from the part of lignified shoot. Total DNA extraction was carried out with CTAB – method.The preparation of samples for high throughput sequencing of the region of 16S rRNA gene was conducted according to Illumina protocol. PCR was carried out on T100 ThermalCycler ("Bio-Rad", USA) with the use of primers reсommended by Illumina, sequencing – on MiSeq (Illumina). Phylogenetic profile of methagenome readings was get with the aid of BLASTN (data base 16S RefSeq Microbial, e-value <0.01) and MEGAN v6.10. 7 independent bacterial communities were analyzed: 2 from plants which grow in MSU Botanical garden, 2 – from plants which grow in Moscow near dwelling houses, 1 – from Moscow region, 2 – from Voronezh industrial zone. It was shown that the most various communities (Shannon-Weaver index = 3.148, 2,32 и 2,304) turned out the communities of endophytic bacteria from the shoots of lilac plants which grew near dwelling houses in Moscow and Moscow region. But on the whole in these communities two genera are predominated: Chthonomonas and Dichotomicrobium. The same ones were found in other analysed communities, while their shares were 3,04-8,07% and 3,02-5,43%, accordingly. The least variety (Shannon-Weaver index =0,813 and 1,385) of endophytic bacteria genera turned out to be in shoots of plants from Voronezh industrial zone. For four bacterial communities, which were obtained from plants growing in MSU Botanical garden and in industrial zone of Voronezh the main genus of bacteria is Burkholderia. Its share is 46,34% and 68% (bacterial communities of plants from MSU botanical garden) and 54,19% and 90,3% (bacterial communities of plants from Voronezh). In bacterial communities of shoots from lilac plants from MSU botanical garden bacteria of genera Sediminibacterium, Ralstonia, Plesiocystis, Microbacterium, Sphingomonas, Pseudomonas and Pseudanabaena were found as well. The representatives of three latest genera also were found in other communities. In whole the composition of bacterial communities of lilac plants from Voronezh turned out to be similar to the bacterial communities of plants from MSU botanical garden. Thus the presence of Chthonomonas, Dichotomicrobium, Sphingomonas, Pseudomonas and Pseudanabaena representatives is characteristic of all analyzed bacterial communities. Three latest genera are typical representatives of leaves and stem microbiota. Chthonomonas and Dichotomicrobium were detected only in plant rhizospheres till quite recently. This work was carried out in accordance to Government order for the Lomonosov Moscow State University (project No.АААА-А16-116021660105-3).
- Добавил в систему: Криницына Анастасия Александровна