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Rapid and homologous immunoassay for the detection of herbicide propisochlor in waterстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 10 августа 2018 г.
- Авторы: Liu Yao, Wang Lan-Teng, Zhou Kai, Eremin Sergei A., Huang Xin-An, Sun Yuan-Ming, Zhen-Lin Xu, Lei Hong-Tao
- Журнал: Food and Agricultural Immunology
- Том: 29
- Номер: 1
- Год издания: 2017
- Издательство: Taylor & Francis
- Местоположение издательства: United Kingdom
- Первая страница: 67
- Последняя страница: 83
- DOI: 10.1080/09540105.2017.1359499
- Аннотация: The herbicide propisochlor can be introduced into the water cycle via agricultural activities, thus possibly leading to harmful ecological effects. It is significant for the development of a rapid method for propisochlor analysis in water. In this work, a rapid homogeneous immunoassay based on fluorescence polarization immunoassay (FPIA) was firstly developed for the determination of propisochlor in pond water. The developed FPIA assay can be completed within only 14 min; the 50% inhibition concentration (IC 50 ) was 125.1 ng/mL and the limit of detection was 8.4 ng/mL. The recoveries of propisochlor in pond water ranged from 80.0% to 86.6%. A high performance liquid chromatography confirmation showed a good correlation with the developed FPIA ( R 2 = 0.9748). The developed FPIA exhibited satisfactory rapidity, high sensitivity and excellent specificity, meeting the standards of the screening assay of propisochlor in pond water on site. The molecular modeling showed that the alkoxyalkyl of chloroacetamide herbicides exerted important effects on the herbicide – antibody binding.
- Добавил в систему: Еремин Сергей Александрович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | 18_fai29_67.pdf | 18_fai29_67.pdf | 2,9 МБ | 30 июня 2018 [Sergei_A_EREMIN] |