Elaboration of a bacterial cellulose matrix for the immobilisation of Escherichia coli cellsстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 24 октября 2018 г.
Аннотация:This paper is concerned with the studies of a natural nanomaterial
which is bacterial cellulose synthesised by Gluconacetobacter hansenii
producer strain. It covers different fields of bacterial cellulose use, including
medicine. The research has proved that bacterial cellulose matrices with
immobilised cells have high potential as immobilisers of cells, including
making probiotics of prolonged action. The matrices consisted of bacterial
cellulose films were prepared by static cultivation of G. hansenii GH-1/2008
strain in the liquid medium. We have developed methods of washing out end
toxins and producer cells of the films in the solutions of sodium bicarbonate,
sodium dodecyl sulphate, and sodium hydroxide. The LAL-test has revealed
that washing the films with sodium dodecyl sulphate is more efficient.
By means of electron scanning and atomic force microscopy (AFM), we
have determined that bacterial cellulose matrices have a layered structure,
smooth surface, and adhesion of E. coli test strain cells. The adhesive capacity,
the energy of adhesion and contact angle is higher for 50 um thick films than
for 20 um thick ones. The bacterial cellulose matrices obtained by the
biosynthesis of G. hansenii strain can be recommended for the immobilisation
of different producer cells.