Efficient DNAfree nanoparticle mediated genome editing of potato using CRISPRCas9 RNP complex

Khromov, A.; Makarov, V.; Makhotenko, A.; Makarova, S.; Snigir, E.; Suprunova, T.; Kalinina, N.; Taliansky, M.

Авторы: Khromov A., Makarov V., Makhotenko A., Makarova S., Snigir E., Suprunova T., Kalinina N., Taliansky M.
Сборник: FEBS Open Bio
Серия: Supplement 1
Том: 8
Тезисы
Год издания: 2018
Место издания: FEBS Open Bio Prague, Czech Republic
Первая страница: 167
Последняя страница: 167
DOI: 10.1002/2211-5463.12453
Аннотация: Due to its simplicity, practicality and efficiency CRISPR–Cas9 is rapidly developing as the emerging technology for genome editing on various eukaryotic systems including higher plants and animals. Here we describe new approach for delivery of preassembled Cas9–sgRNA ribonucleoproteins into dissected potato (Solanum tuberosum, cv Chicago) shoot apical meristems by chitosan nanoparticles using vacuum infiltration and regeneration of plants with edited alleles. As a model gene for genome editing, we have selected a gene encoding coilin, a major protein of Cajal bodies(CBs). In additional to its traditional role in assembly of CBs, coilin also participates in biotic (virus attack) and abiotic (high salinity) plant stress responses. Using this method of delivery, we have demonstrated DNAfree genome editing and recovery of potato plants with mutated (disrupted) coilin gene. The generated edited plants exhibit high level of resistance to potato virus Y and significant tolerance to osmotic and salt stress. This work opens up a new avenue for practical application of CRISPRCas9 technology to produce genome edited (transgenefree) potato plants and other crops. The present work was performed with the financial support from the Russian Science Foundation (grant № 161604019).
Добавил в систему: Макарова Светлана Сергеевна

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