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New Strains of Hydrocarbon Oxidizing Bacteria from Lake Beloeстатья
- Авторы: Kravzova T.R., Lazebnaya I.V., Sidorov R.A., Lazebny O.E., Ilinsky V.V., Chekanov K.A., Komarova T.I., Akulova A.Yu, Mosharova I.V., Koksharova O.A.
- Сборник: Molecular Phylogenetics: Contributions to the 5th Moscow International Conference «MOLECULAR PHYLOGENETICS AND BIODIVERSITY BIOBANKING» (Moscow, Russia, August 25-28, 2018) / Eds. A. Troitsky and L. Rusin
- Глава в коллективной монографии
- Год издания: 2018
- Место издания: TORUS PRESS Moscow, Russian Federation
- Первая страница: 81
- Последняя страница: 82
- DOI: 10.30826/MolPhy2018-51
- Аннотация: The problem of environment pollution generates the needs in bioremediation. New oil degrading bacterial strains 2012B and 2012C were isolated from the Lake Beloe, one of three ice Kosinskie Lakes in Moscow. Molecular identification of the bacterial strains was performed by using 16S rRNA gene sequence analysis (GenBank: KP779654.1, MG966152) and in case of 2012B strain additionally alkB partial gene sequence (KR422620.1) was used. Phylogenetic reconstructions were performed by application of different methods (BIONJ, PhyML, DNAPars, MRBayes, and GARLI_ML). Comparison of rRNA gene sequences shows that 2012B strain belongs to genus Rhodococcus. Strain 2012B formed a coherent cluster with R. erythropolis NVI 00/50/667, R. qingshengii dj1-6, R. jialingiae dj1-6-2 and R.erythropolis DSM 43066T. Phylogenetic analysis based on the alkB gene sequence placed strain 2012B in one cluster with R. qingshengii strain BLH-Y4 (KF696707). The morphological and chemotaxonomic properties of the isolate 2012B were typical for members of the genus Rhodococcus. Lipids of the strain 2012B were comprised of C14:0-C19:0 fatty acids, where the most abundant were C15:0 (54%), C16:0 (17%) and C17:0 (10%). Phylogenetic analysis by using 16S rRNA gene sequence revealed that the strain 2012C (GenBank: MG966152) has the highest identity and forms one cluster with Pseudomonas spp. (P. oleovorans, P. oryzihabitans, P. psychrotolerans, P. aeruginasa). Lipids of the strain 2012C were comprised of C14:0-C19:0 fatty acids, where the most abundant were C15:0 (45%), C16:0 (32%) and C17:0 (9%). These new bacterial strains could be useful in future bioremediation experiments and in biotechnological applications.
- Добавил в систему: Кокшарова Ольга Алексеевна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | MOLPHY-5-Koksharova_1.pdf | MOLPHY-5-Koksharova_1.pdf | 208,7 КБ | 26 сентября 2018 [oa-koksharova@rambler.ru] | |
| 2. | Kravzova_2018b.pdf | Kravzova_2018b.pdf | 76,1 КБ | 6 сентября 2018 [oa-koksharova@rambler.ru] |