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Molecular Phylogeny and Physiological Features of a New Nanodiamond Resistant Bacteriumстатья
- Авторы: Kravzova T.R., Safronova N.A., Sidorov R.A., Koksharova O.A.
- Сборник: Molecular Phylogenetics: Contributions to the 5th Moscow International Conference «MOLECULAR PHYLOGENETICS AND BIODIVERSITY BIOBANKING» (Moscow, Russia, August 25-28, 2018) / Eds. A. Troitsky and L. Rusin
- Глава в коллективной монографии
- Год издания: 2018
- Место издания: TORUS PRESS Moscow, Russian Federation
- Первая страница: 83
- Последняя страница: 84
- DOI: 10.30826/MolPhy2018-52
- Аннотация: Detonation nanodiamonds (NDs) are engineered nanoparticles used in a wide range of commercial applications. Ending up in the environment NDs entail a potential hazard for living organisms. A new bacterial strain that is resistant to high concentrations of NDs was purified and characterized. It was identified as Rhodococcus sp strain SR 2016 (hereafter, SR 2016) by using the 16S rRNA gene sequence (GenBank: MG561935). Comparison of this gene sequence with all the GenBank sequences showed that the SR2016 does not have 100 percent identity with any other organisms investigated previously. The GenBank sequences of selected species were aligned by using the algorithm Muscle (MEGA 6.0). Phylogenetic reconstructions were performed by using Bayesian inference (MrBayes ver. 3.1.2). Lipids in SR 2016 comprise a range of C14:0 - C19:0 fatty acids, where C15:0 (45%), C16:0 (28%) and C17:0 (13%) fatty acids predominant. The new strain was studied by scanning electron microscopy and physiology methods. It was shown that strain SR 2016 utilizes: l-inosine, l-arabinose, l-asparagine, d-maltose, and d-sorbitol and does not utilize: benomyl and carbendazim. Strain SR 2016 remained resistant to all tested NDs concentrations (0.25, 0.5, 5, 10 mg/mL). It was found that in the absence of other alternative sources of carbon, the presence of even low amounts of NDs allows SR 2016 to maintain its vitality in a mineral medium. Moreover, high NDs concentrations in the presence of a readily available source of carbon in the medium increase the abundance of viable cells of SR 2016. Thereby, bacterium Rhodococcus sp. strain SR 2016 could be considered as a potential agent for bioremediation of NDs.
- Добавил в систему: Кокшарова Ольга Алексеевна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | MOLPHY-5-Koksharova_1.pdf | MOLPHY-5-Koksharova_1.pdf | 208,7 КБ | 26 сентября 2018 [oa-koksharova@rambler.ru] | |
| 2. | Kravzova_2018a.pdf | Kravzova_2018a.pdf | 73,2 КБ | 6 сентября 2018 [oa-koksharova@rambler.ru] |