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Intrinsic Fluorescence of Metabolite Amyloids Allows Label-Free Monitoring in Live Cellsстатья Исследовательская статья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Shaham-Niv Shira, Arnon Zohar A., Sade Dorin, Lichtenstein Alexandra, Shirshin Evgeny A., Kolusheva Sofiya, Gazit Ehud
- Журнал: Angewandte Chemie - International Edition
- Год издания: 2018
- Издательство: John Wiley & Sons Ltd.
- Местоположение издательства: United Kingdom
- DOI: 10.1002/anie.201806565
- Аннотация: The formation of apoptosis‐inducing amyloidal structures by metabolites has significantly extended the "amyloid hypothesis" to include non‐proteinaceous, single metabolite building blocks. However, detection of metabolite assemblies is restricted, as compared to their larger protein‐based counterparts, due to the hindrance of external labelling and limited immunohistochemistry detection tools. Here, we present the detection of the formation, dynamics and cellular distribution of metabolite amyloid‐like structures and provide mechanistic insights into the generation of supramolecular chromophores. Moreover, the intrinsic fluorescence properties allow the detection of metabolite assemblies in living cells without the use of any external dye. Altogether, this intrinsic fluorescence of metabolite assemblies further verify their amyloidal nature, while providing an important tool for further investigation of their pathological role in inborn error of metabolism disorders.
- Добавил в систему: Ширшин Евгений Александрович