Inactivation of planktonic escherichia coli by focused 1-MHz ultrasound pulses with shocks: efficacy and kinetics upon volume scale-up

Brayman, A.A.; Macconaghy, B.E.; Wang, Y.N.; Chan, K.T.; Monsky, W.L.; Chernikov, V.P.; Buravkov, S.V.; Khokhlova, V.A.; Matula, T.J.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 30 августа 2018 г.

Авторы: Brayman A.A., Macconaghy B.E., Wang Y.N., Chan K.T., Monsky W.L., Chernikov V.P., Buravkov S.V., Khokhlova V.A., Matula T.J.
Журнал: Ultrasound in Medicine and Biology
Том: 44
Номер: 9
Год издания: 2018
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Первая страница: 1996
Последняя страница: 2008
DOI: 10.1016/j.ultrasmedbio.2018.05.010
Аннотация: This study addresses inactivation of E. coli in either 5 or 10 mL volumes, which were 50- to 100-fold greater than used in an earlier study (Brayman et al. 2017). Cells were treated with 1-MHz pulsed HIFU (10 cycles, 2 kHz repetition frequency, +65/-12.8 MPa focal pressures). Surviving fraction [SF] was assessed by coliform assay, and inactivation demonstrated curvilinear kinetics. Reduction of SF to 50% required 2.5 or 6 min in 5 or 10 mL samples, respectively. Exposure of 5 mL for 20 min reduced SF to ~1%; similar exposure of 10 mL samples reduced SF to ~10%. Surviving cells from 5 min exposures appeared normal under light microscopy, with minimal debris; after 20 min, debris dominated. TEM images of insonated samples showed some undamaged cells, a few damaged but largely intact cells, and comminuted debris. Cellular damage associated with substantive but incomplete levels of inactivation can be variable, ranging from membrane holes tens of nanometers in diameter, to nearly complete comminution.
Добавил в систему: Хохлова Вера Александровна

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Inactivation of planktonic escherichia coli by focused 1-MHz ultrasound pulses with shocks: efficacy and kinetics upon volume scale-upстатья

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