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DNA duplexes containing 2'-deoxy-2'-iodoacetamidouridine as reagents for affinity modification of proteinsстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Borisova O.A., Romanenkov A.S., Metelev V.G., Kubareva E.A., Zubin E.M., Timchenko M.A., Oretskaya T.S.
- Журнал: Molecular Biology
- Том: 37
- Номер: 3
- Год издания: 2003
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 456
- Последняя страница: 463
- DOI: 10.1023/A:1024203830741
- Аннотация: DNA duplexes containing the iodoacetamido group at position 2' of the ribose moiety were proposed for affinity modification of Cys in DNA-binding proteins. Reactive DNA derivatives were obtained with iodoacetic anhydride and synthetic oligodeoxyribonucleotides containing 2'-amino-2'-deoxyuridine in place of thymine at various positions. The derivatives were tested for reaction with amino acids and peptides and shown to specifically interact with Cys-containing proteins. The possibility of using the modified DNA duplexes to probe the protein SH group close to the DNA sugar-phosphate backbone in DNA-protein complexes was demonstrated with the example of subunit p50 of human transcription factor NF-kappa B.
- Добавил в систему: Орецкая Татьяна Семеновна