Synthesis and properties of a covalently linked DNA duplex containing a recognition site for transcription factor NF-kappa Bстатья
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:A DNA duplex with a recognition site for transcription factor NF-kappa B was linked in terminal phosphates with a series of ''bridging agents'' carrying two or more aliphatic amino groups, using 1-ethyl-3-(3'-dimethylaminopropyl)carbodiimide condensation. The best results were obtained with polymyxin M: the linked duplex had enhanced heat stability, was nuclease-resistant, and formed a specific complex with the NF-kappa B p50 subunit, whereby the apparent association constant was twice greater than for a nonlinked duplex.