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Formation of stable triplexes between purine RNA and pyrimidine oligodeoxyxylonucleotidesстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Ivanov S., Alekseev Y., Bertrand J.R., Malvy C., Gottikh M.B.
- Журнал: Nucleic Acids Research
- Том: 31
- Номер: 14
- Год издания: 2003
- Издательство: Oxford University Press
- Местоположение издательства: United Kingdom
- Первая страница: 4256
- Последняя страница: 4263
- DOI: 10.1093/nar/gkg443
- Аннотация: Hybridization properties of oligodeoxyxylonucleotides (OXNs) built from pyrimidine monomers with an inverted 3’-OH group of the furanose have been studied using the gel mobility shift, UV melting and circular dichroism (CD) spectroscopy methods. Pyrimidine OXNs form triple helices with complementary purine RNA in which one OXN is parallel and another is antiparallel with respect to the RNA target. Surprisingly, no duplex formation between the pyrimidine OXNs and purine RNAs is detected. The modified triplexes are stable at pH 7. Their thermal stability depends on the number of C(G-C) triplets and, for G-rich RNA sequences, it is comparable with the stability of native DNA-RNA duplexes. The CD spectra of triplexes formed by OXNs with purine RNA targets are similar to spectra of A-type helices. A pyrimidine OXN having a clamp structure efficiently inhibits reverse transcription of murine pim-1 mRNA in vitro mediated by the Mo-MuLV reverse transcriptase.
- Добавил в систему: Готтих Марина Борисовна