Characterization of the Structure of the Prokaryotic Complex of Antarctic Permafrost by Molecular Genetic Techniques

Manucharova, N.A.; Trosheva, E.V.; Kol’tsova, E.M.; Demkina, E.V.; Karaevskaya, E.V.; Rivkina, E.M.; Mardanov, A.V.; El’-Registan, G.I.

Авторы: Manucharova N.A., Trosheva E.V., Kol’tsova E.M., Demkina E.V., Karaevskaya E.V., Rivkina E.M., Mardanov A.V., El’-Registan G.I.
Журнал: Microbiology
Том: 85
Номер: 1
Год издания: 2016
Издательство: Pleiades Publishing, Ltd
Местоположение издательства: Road Town, United Kingdom
Первая страница: 102
Последняя страница: 108
Оригинальные версии: ХАРАКТЕРИСТИКА СТРУКТУРЫ ПРОКАРИОТНОГО КОМПЛЕКСА МНОГОЛЕТНЕМЕРЗЛЫХ ГРУНТОВ АНТАРКТИДЫ НА ОСНОВАНИИ ДАННЫХ МОЛЕКУЛЯРНО-БИОЛОГИЧЕСКИХ МЕТОДОВ
DOI: 10.1134/S0026261716010057
Аннотация: A prokaryotic mesophilic organotrophic community responsible for 10% of the total microbial number determined by epifluorescence microscopy was reactivated in the samples of Antarctic permafrost retrieved from the environment favoring long-term preservation of microbial communities (7500 years). No culturable forms were obtained without resuscitation procedures (CFU = 0). Proteobacteria, Actinobacteria, and Firmicutes were the dominant microbial groups in the complex. Initiation of the reactivated microbial complex by addition of chitin (0.1% wt/vol) resulted in an increased share of metabolically active biomass (up to 50%) due to the functional domination of chitinolytics caused by the target resource. Thus, sequential application of resuscitation procedures and initiation of a specific physiological group (in this case, chitino- lytics) to a permafrost-preserved microbial community made it possible to reveal a prokaryotic complex capa- ble of reversion of metabolic activity (FISH data), to determine its phylogenetic structure by metagenomic analysis, and to isolate a pure culture of the dominant microorganism with high chitinolytic activity. The work was supported by grant RSF No 14-50-00029 Keywords: permafrost soils, Antarctica, metagenomic analysis, f luorescent in situ hybridization (FISH), met- abolically active prokaryotic complex.
Добавил в систему: Манучарова Наталия Александровна

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Characterization of the Structure of the Prokaryotic Complex of Antarctic Permafrost by Molecular Genetic Techniquesстатья

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