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Lectins: new approach to their sensing and isolationстатья
Информация о цитировании статьи получена из
Scopus
Дата последнего поиска статьи во внешних источниках: 3 июля 2019 г.
- Авторы: Samoilova N.A., Krayukhina M.A., Likhosherstov L.M., Yamskov I.A., Piskarev V.E.
- Сборник: Advances in Chemistry Research
- Том: 41
- Глава в коллективной монографии
- Год издания: 2017
- Место издания: Nova Science Publisher New York
- Первая страница: 91
- Последняя страница: 144
- Аннотация: Abstract Lectins (agglutinins) are specific carbohydrate-binding proteins of non- immune origin. Lectin –carbohydrate interactions play a key role in a great number of vital biological processes. Rapid detection and isolation of various lectin types is required for diagnosis and therapeutics. Simple, convenient, and inexpensive method of preparation of lectin specific nanogold (silver)-labeled colloidal, and cross-linked neoglycoconjugates, based on maleic anhydride copolymers, was developed. Water-soluble nanogold (silver)-labeled glyconanoparticles were obtained in two steps: i) introduction of free N-acetyl-D- glucosamine, or glycyl-spacered glycosynthons – β-N-glycyl-N-acetyl-D-glucosamine, or β- N-glycyl-lactose, – into poly(ethylene-alt-maleic anhydride), or poly(N-vinylpyrrolidone- alt-maleic anhydride), and ii) labeling in situ thus obtained glycopolymers with gold or silver nanoparticles. Lectin sorbents were synthesized from glycyl-spacered glycosynthons and spherical granulated cross-linked maleic anhydride copolymers in aqueous system without any condensing agents. Thus, maleic anhydride copolymer was used solely, without any preliminary modification of the polymer, as a matrix for cross-linking, for specific ligand binding, and as metal nanoparticles stabilizing cover. Resulted colloidal gold or silver glyconanoparticles were used as lectin sensors (in dot-blot analysis of lectins); cross-linked neoglycoconjugates – for lectin sorption studies. The correspondent gold or silver labeled water-soluble glyconanoparticles, and cross- linked neoglycoconjugates, manifested high activity and specificity in all tests with series of β-D-GlcNAc-specific and β-D-Gal-specific lectins.
- Добавил в систему: Краюхина Мария Александровна