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Mistletoe lectin dissociates into catalytic and binding subunits before translocation across the membrane to the cytoplasmстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Agapov II, Tonevitsky AG, Moysenovich MM, Maluchenko NV, Kirpichnikov MP
- Журнал: FEBS Letters
- Том: 452
- Номер: 3
- Год издания: 1999
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 211
- Последняя страница: 214
- DOI: 10.1016/S0014-5793(99)00639-0
- Аннотация: Hybridomas producing monoclonal antibodies (mAbs) against the mistletoe lectin A-chain (MLA) were obtained to investigate the intracellular routing and translocation of ribosome-inactivating proteins. Anti-MLA mAb MNA5 did not bind the holotoxin but interacted with isolated MLA. This epitope was not recognized upon MLA denaturation or conjugation of MLA with the ricin binding subunit (RTB). Furthermore, the mAbs did not appreciably react with a panel of MLA synthetic octapeptides linked to the surface of polyethylene pins. A study of the cytotoxicity of mistletoe lectin, ricin, and chimeric toxin MLA/RTB for the hybridomas revealed that interchain disulfide bond reduction and subunit dissociation are required for cytotoxic activity of mistletoe lectin.
- Добавил в систему: Малюченко Наталия Валериевна