Photodynamic action as a basis for relaxation method of the study of gramicidin channelsстатья
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Статья опубликована в журнале из перечня ВАК
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 29 мая 2015 г.
Аннотация:Sensitized photoinactivation of ionic channels formed by gramicidin A in bilayer lipid membranes is reviewed. There has been a long way from the discovery of this phenomenon in 1992 and the first ideas concerning its mechanism till its application for studying the interaction of different agents with modified gramicidins. Measuring transmembrane current transients after a flash of visible light in the presence of photosensitizer proves to be very convenient method of determining rate constants of gramicidin channel formation and dissociation. In the present review, both the advantages and the drawbacks of the photoinactivation technique are compared with those of the conventional approaches, such as single-channel measurements, the voltage-jump technique, and the noise power spectrum analysis. A study of interaction of charged gramicidin analogues with polyelectrolytes by using the sensitized photoinactivation procedure is described here in detail to demonstrate the wide potencies of this method. In addition, the application of ionic channels as components of sensor devices is briefly reviewed.