Glaciibacter superstes gen. nov., sp nov., a novel member of the family Microbacteriaceae isolated from a permafrost ice wedge

Katayama, T.; Kato, T.; Tanaka, M.; Douglas, T.A.; Brouchkov, A.; Fukuda, M.; Tomita, F.; Asano, K.

Авторы: Katayama Taiki, Kato Tomoko, Tanaka Michiko, Douglas Thomas A., Brouchkov Anatoli, Fukuda Masami, Tomita Fusao, Asano Kozo
Журнал: International Journal of Systematic and Evolutionary Microbiology
Том: 59
Год издания: 2009
Издательство: Society for General Microbiology
Местоположение издательства: United Kingdom
Первая страница: 482
Последняя страница: 486
DOI: 10.1099/ijs.0.001354-0
Аннотация: Gram-positive, aerobic, non-spore-forming, irregular rod-shaped bacteria, designated strains AHU1791T and AHU1810, were isolated from a permafrost ice wedge in Alaska. Cells were motile by means of a polar flagellum. The strains were psychrophilic, growing at −5 to 25 °C. Phylogenetic analysis of 16S rRNA gene sequences indicated that the ice-wedge isolates formed a clade distinct from other genera affiliated with the family Microbacteriaceae. The novel strains showed highest levels of 16S rRNA gene sequence similarity with members of the genera Agreia and Subtercola (95.6–95.9 %). The level of 16S rRNA gene sequence similarity between strains AHU1791T and AHU1810 was 99.8 %. The cell-wall peptidoglycan type of the two strains was B2γ, containing 2,4-diaminobutyric acid as the diagnostic amino acid. The predominant menaquinones were MK-12 and MK-13 (strain AHU1791T) and MK-11 and MK-12 (strain AHU1810). The major fatty acids of the two strains were 12-methyl tetradecanoic acid (anteiso-C15 : 0), 14-methyl hexadecanoic acid (anteiso-C17 : 0), 14-methyl pentadecanoic acid (iso-C16 : 0) and 13-methyl tetradecanoic acid (iso-C15 : 0). The DNA G+C contents of strains AHU1791T and AHU1810 were approximately 65 mol%. These phenotypic characteristics differentiated the ice-wedge strains from their closest phylogenetic neighbours, namely Subtercola boreus and the two recognized species of the genus Agreia. The sequences of the housekeeping genes coding for DNA gyrase subunit B (gyrB), RNA polymerase subunit B (rpoB) and recombinase A (recA) were almost identical between strains AHU1791T and AHU1810. Although the predominant menaquinones found in strains AHU1791T and AHU1810 were different, no other distinct differences were found with regard to other phenotypic and genotypic characteristics, indicating that the two strains were members of the same species. Accordingly, strains AHU1791T and AHU1810 are considered to represent a single novel species of a new genus, for which the name Glaciibacter superstes gen. nov., sp. nov. is proposed. The type strain of Glaciibacter superstes is AHU1791T (=DSM 21135T =NBRC 104264T). The GenBank/EMBL/DDBJ accession numbers of the 16S rRNA, gyrB, rpoB and recA gene sequences of strains AHU1791T and AHU1810 are AB378301 and AB378302 (16S rRNA), AB436916 and AB436917 (gyrB), AB436918 and AB436919 (rpoB) and AB436920 and AB436921 (recA), respectively.
Добавил в систему: Брушков Анатолий Викторович

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Glaciibacter superstes gen. nov., sp nov., a novel member of the family Microbacteriaceae isolated from a permafrost ice wedgeстатья

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