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Synthetic CpG oligonucleotides as potential modulators of neutrophil survival in PAMP‐associated inhibition of apoptosisстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 14 августа 2019 г.
- Авторы: Golenkina E.A., Viryasova G.M., Galkina S.I., Arifulin E.A., Gaponova T.V., Romanova Y.M., Sud'ina G.F.
- Журнал: Journal of Leukocyte Biology
- Том: 106
- Номер: 1
- Год издания: 2019
- Издательство: Federation of American Societies for Experimental Biology
- Местоположение издательства: United States
- Первая страница: 45
- Последняя страница: 55
- DOI: 10.1002/JLB.3MIA1118-435R
- Аннотация: We studied the effects of a synthetic CpG oligonucleotide (CpG ODN2006) on polymorphonuclear leukocyte (PMNL, neutrophil) survival and oxidant status. CpG ODN2006 showed a dose‐dependent effect on the apoptosis of resting neutrophils. Without affecting the viability of resting cells, low concentrations of CpG ODN2006 interfered with Salmonella typhimurium‐mediated viability prolongation and increased neutrophil apoptosis to control levels. CpG ODN2006 stimulated neutrophil apoptosis by enhancing ROS generation. Even small doses of ODN could induce the production of intracellular superoxide anions. The high superoxide reactogenicity, including with respect to nitrogen oxide, led to increased levels of intracellular ROS and RNS, which ultimately caused apoptosis. The pro‐oxidant effect of low concentrations of CpG ODN2006 was not sufficient to trigger irreversible pro‐apoptotic mechanisms. However, the sensitivity of PMNLs to ODN2006, a modulator of apoptosis, increased significantly under conditions of infectious inflammation. Inactivated S. typhimurium proved to be suitable for simulating inflammatory conditions in vitro.
- Добавил в систему: Судьина Галина Федоровна