Towards embedding Caco-2 model of gut interface in a microfluidic device to enable multi-organ models for systems biology

Sakharov, D.; Maltseva, D.; Knyazev, E.; Nikulin, S.; Poloznikov, A.; Shilin, S.; Baranova, A.; Tsypina, I.; Tonevitsky, A.

Авторы: Sakharov Dmitry, Maltseva Diana, Knyazev Evgeny, Nikulin Sergey, Poloznikov Andrey, Shilin Sergey, Baranova Ancha, Tsypina Irina, Tonevitsky Alexander
Журнал: BMC Systems Biology
Том: 13
Номер: Suppl 1
Год издания: 2019
Издательство: BioMed Central
Местоположение издательства: London
Первая страница: 19
DOI: 10.1186/s12918-019-0686-y
Аннотация: BACKGROUND: A cancer cell line originating from human epithelial colorectal adenocarcinoma (Caco-2 cells) serves as a high capacity model for a preclinical screening of drugs. Recent need for incorporating barrier tissue into multi-organ chips calls for inclusion of Caco-2 cells into microperfused environment. RESULTS: This article describes a series of systems biology insights obtained from comparing Caco-2 models cells grown as conventional 2D layer and in a microfluidic chip. When basic electrical parameters of Caco-2 monolayers were evaluated using impedance spectrometry and MTT assays, no differences were noted. On the other hand, the microarray profiling of mRNAs and miRNAs revealed that grows on a microfluidic chip leads to the change in the production of specific miRNA, which regulate a set of genes for cell adhesion molecules (CAMs), and provide for more complete differentiation of Caco-2 monolayer. Moreover, the sets of miRNAs secreted at the apical surface of Caco-2 monolayers grown in conventional 2D culture and in microfluidic device differ. CONCLUSIONS: When integrated into a multi-tissue platform, Caco-2 cells may aid in generating insights into complex pathophysiological processes, not possible to dissect in conventional cultures. KEYWORDS: 2D layer; Caco-2 cell lines; Microfluidic chip; On-the-chip models
Добавил в систему: Князев Евгений Николаевич

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Towards embedding Caco-2 model of gut interface in a microfluidic device to enable multi-organ models for systems biologyстатья

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