Calcium-induced calcium release mediates all-or-nothing responses of mesenchymal stromal cells to noradrenaline

Kotova, P.D.; Turin-Kuzmin, P.A.; Rogachevskaja, O.A.; Fadeeva, J.I.; Sysoeva, V.Y.; Tkachuk, V.A.; Kolesnikov, S.S.

Авторы: Kotova P.D., Turin-Kuzmin P.A., Rogachevskaja O.A., Fadeeva J.I., Sysoeva V.Yu, Tkachuk V.A., Kolesnikov S.S.
Журнал: Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology
Том: 8
Номер: 1
Год издания: 2014
Издательство: Pleiades Publishing, Ltd
Местоположение издательства: Road Town, United Kingdom
Первая страница: 82
Последняя страница: 88
DOI: 10.1134/s1990747813050085
Аннотация: By using Ca2+ imaging and Fluo4 dye, we examined the capability of certain agonists of Gprotein coupled receptors to stimulate Ca2+ signaling in cultured mesenchymal stromal cells (MSC) derived from the human adipose tissue. In particular, a small subpopulation (~5%) MSC was found to respond to noradrenaline with Ca2+ transients. The allornothing fashion was characteristic of adrenergic Ca2+ signaling in MSC, that is, while at low concentrations noradrenaline stimulated undetectable Ca2+ transients, virtually maximal responses were elicited by this agonist at any concentration above the threshold of 100–200 nM. In some experiments, MSC were loaded with the photosensitive Ca2+ chelator NPEGTA to produce local or global jumps in cytosolic Ca2+ concentration by virtue of Ca2+ uncaging. Global uncaging eliciting a high enough Ca2+ jump triggered a Ca2+ transient in the MSC cytoplasm, which was similar to a noradrenaline response kinetically and by magnitude. When Ca2+ uncaging was produced locally, it initiated a Ca2+ signal that traveled along a cell with a speed that exceeded an expected one by two orders of magnitude, should Ca2+ signal transfer be mediated merely by passive Ca2+ diffusion in the presence of Ca2+ buffer. These findings implicated Ca2+induced Ca2+ release (CICR) as a mechanism amplifying local Ca2+ signals in MSC. Of Ca2+ targets involved in CICR, the ryanodine receptor and IP3 receptor are only known. The inhibitory analysis revealed IP3 receptors to be principally responsible for CICR in MSC, whereas a contribution of ryanodine receptors was negligible. Altogether, our results suggest that an initial noradrenalinedependent rise in cytosolic Ca2+ stimulates, should it reach the threshold level, IP3 receptors, thereby triggering an avalanche like Ca2+ release from Ca2+ stores and underlying the allornothing dependence of cellular responses on the agonist concentration.
Добавил в систему: Тюрин-Кузьмин Петр Алексеевич

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Calcium-induced calcium release mediates all-or-nothing responses of mesenchymal stromal cells to noradrenalineстатья

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