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An Adiabatic Calorimetry Method to Determine the Thermodynamic Characteristics of Cryoprotectantsстатья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 11 ноября 2019 г.
- Авторы: Simonenko E.Yu, Pryadun V.V., Ivanova A.A., Burmistrova E., Vasiliev A., Yakovenko S.A.
- Журнал: Biophysics
- Том: 64
- Номер: 1
- Год издания: 2019
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 1
- Последняя страница: 6
- DOI: 10.1134/S0006350919010172
- Аннотация: Abstract—The efficiency of cryoprotectants used to protect cells from damage is usually evaluated by thechanges in vital cell parameters after a freezing–thawing cycle. Certain physical parameters, such as the glass transition temperature, viscosity, toxicity, and the minimum concentration necessary for vitrification, are known for several components of cryoprotectants. However, it is impossible to provide physicochemical characteristics for a medium that contains both penetrating and nonpenetrating cryoprotective agents. An adiabatic calorimetry method adapted to studying liquid media was used to describe the temperature depen-dence of the heat capacity and to find the temperatures of the phase transition and changes in the state of aggregation for a glycerol-containing solution, which is commonly used as a basic component of cryoprotectants, and for a commercial cryoprotectant. Changes in entropy and enthalpy in the commercial cryoprotectant were 1.5 times higher than in aqueous solutions of glycerol.
- Добавил в систему: Симоненко Екатерина Юрьевна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | simonenko2019.pdf | simonenko2019.pdf | 630,7 КБ | 19 ноября 2020 [SAYakovenko] |