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Coprecipitation of proteins and DNA-protein complexes with magnesium sarcolysate: a study of an association of topoisomerase I and RNA polymerase with DNAстатья
- Авторы: VASSETZKY Y.S., Bakayev V.V.
- Журнал: Biology bulletin of the Academy of Sciences of the USSR
- Том: 1
- Год издания: 1988
- Издательство: Consultants Bureau
- Местоположение издательства: United States
- Первая страница: 166
- Последняя страница: 169
- Аннотация: A specific cosedimentation of proteins and their complexes with DNA at low temperature (M-band technique) has been demonstrated. Model experiments with reconstituted SV40 DNA-topoisomerase I and SV40 DNA-E. coli RNA polymerase complexes demonstrated the potential and capacities of the method. It allows fractionation of DNA-protein complexes from naked DNA and has greater range, higher reproducibility and lower background values than similar methods.
- Добавил в систему: Васецкий Егор Сергеевич