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Generation of uncultivable forms of Lactobacillus plantarum 8R-A3 under solid-state cultivation on wheat branстатья Исследовательская статья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 17 июля 2019 г.
- Авторы: Ushakova N.A., Abramov V.M., Khlebnikov V.S., Semenov A.M., Kuznetsov B.B., Kozlova A.A., Nifatov A.V., Sakulin V.K., Kosarev I.V., Vasilenko R.N., Sukhacheva M.V., Melnikov V.G.
- Журнал: Biology Bulletin
- Том: 39
- Номер: 6
- Год издания: 2012
- Издательство: M A I K Nauka - Interperiodica
- Местоположение издательства: Russian Federation
- Первая страница: 525
- Последняя страница: 533
- DOI: 10.1134/S1062359012050159
- Аннотация: This paper shows that in the solid-state cultivation of Lactobacillus plantarum 8R-A3 on wheat bran, a biofilm was formed on the surface of the carrier within 48 h. Prolongation of fermentation caused a drop in the number of CFU from 96% of the initial total number of cells to 8.8% by 72 h. When the temperature was raised from 37 to 45°C, which led to drying of the fermentation mass, the CFU index decreased to <104. According to fluorescence microscopy data, up to 40% of bacteria with signs of life survived in the dry specimens. After keeping the mice on a diet with the introduction of 0.05% of fermented bran dried for 72 hours, a strain genetically identical to the L. plantarum 8R-A3 was extracted from the colon. In animals with amikacin-inhibited intestinal lactobacilli, their normal level recovered. It is suggested that L. plantarum 8R-A3 generates uncultivable forms, which are reanimated by passage through the animal organism and exhibit probiotic activity. The biofilm formed in the solid-state cultivation contributes to the survival of lactobacilli cells in drying of the fermentation mass.
- Добавил в систему: Семенов Александр Михайлович