|
ИСТИНА |
Войти в систему Регистрация |
ФНКЦ РР |
||
Efficient RNA silencing suppression activity of Potato Mop-Top Virus 8K protein is driven by variability and positive selectionстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 26 сентября 2019 г.
- Авторы: Kalyandurg Pruthvi B., Tahmasebi Aminallah, Vetukuri Ramesh R., Kushwaha Sandeep K., Lezzhov Alexander A., Solovyev Andrey G., Grenville-Briggs Laura J., Savenkov Eugene I.
- Журнал: Virology
- Том: 535
- Год издания: 2019
- Издательство: Academic Press
- Местоположение издательства: United States
- Первая страница: 111
- Последняя страница: 121
- DOI: 10.1016/j.virol.2019.06.018
- Аннотация: Previously, we investigated the evolution of Potato mop-top virus (PMTV) ORFs. Results indicate that positive selection acts exclusively on an ORF encoding the 8K protein, a weak viral suppressor of RNA silencing (VSR). However, how the extraordinary variability contributes to 8K-mediated RNA silencing suppression remains unknown. Here, we characterized the RNA silencing suppression activity of the 8K protein from seven diverse isolates. We show that 8K encoded by isolate P1 exhibits stronger RNA silencing suppression activity than the 8K protein from six other isolates. Mutational analyses revealed that Ser-50 is critical for these differences. By comparing small RNA profiles we found a lower abundance of siRNAs with U residue at the 5′-terminus after expression of the P1 8K compared to expression of 8K from isolate P125, an isolate with weak VSR activity. These results provide new clues as to the role of positive selection in shaping activities of VSRs.
- Добавил в систему: Лезжов Александр Александрович