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[The critical significance of a conserved single-stranded interdomain linker in the 5'-untranslated region of poliovirus RNA]статья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 28 мая 2015 г.
- Авторы: Belov G.A., Gmyl A.P., Maslova S.V., Pilipenko E.V., Agol V.I.
- Журнал: Molekuliarnaia biologiia
- Том: 29
- Номер: 2
- Год издания: 1995
- Издательство: Russian Academy of Sciences
- Местоположение издательства: Russian Federation
- Первая страница: 294
- Последняя страница: 300
- Аннотация: Translation of polioviral RNA is initiated by interaction of a small ribosomal subunit with internal segments of the 5'-untranslated region (5'UTR). Several mutations were constructed within 5'UTR segment 425-449. All of them (including a single C444-->U replacement) inhibited in vitro translation, which decreased about 10-fold. Two mutant constructs, pPV12-05 (C444-->U) and pPV12K (containing also an AAUU insert between positions 441 and 442) produced plaques on monolayers of susceptible cells. All the viruses isolated from these plaques exhibited a reversion at position 444; the template activities of the revertant RNAs were restored completely or significantly. The results show the importance of the relevant 5'UTR segment for the initiation of polioviral RNA translation.
- Добавил в систему: Гмыль Анатолий Петрович