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A novel antifungal hevein-type peptide from Triticum kiharae seeds with a unique 10-cysteine motifстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 20 июня 2016 г.
- Авторы: Odintsova Tatyana I., Vassilevski Alexander A., Slavokhotova Anna A., Musolyamov Alexander K., Finkina Ekaterina I., Khadeeva Natalia V., Rogozhin Eugene A., Korostyleva Tatyana V., Pukhalsky Vitalii A., Grishin Eugene V.
- Журнал: FEBS Journal
- Том: 276
- Номер: 15
- Год издания: 2009
- Издательство: Blackwell Publishing Inc.
- Местоположение издательства: United Kingdom
- Первая страница: 4266
- Последняя страница: 4275
- DOI: 10.1111/j.1742-4658.2009.07135.x
- Аннотация: Two forms of a novel antimicrobial peptide (AMP), named WAMP-1a and WAMP-1b, that differ by a single C-terminal amino acid residue and belong to a new structural type of plant AMP were purified from seeds of Triticum kiharae Dorof. et Migusch. Although WAMP-1a and WAMP-1b share similarity with hevein-type peptides, they possess 10 cysteine residues arranged in a unique cysteine motif which is distinct from those described previously for plant AMPs, but is characteristic of the chitin-binding domains of cereal class I chitinases. An unusual substitution of a serine for a glycine residue in the chitin-binding domain was detected for the first time in hevein-like polypeptides. Recombinant WAMP-1a was successfully produced in Escherichia coli. This is the first case of high-yield production of a cysteine-rich plant AMP from a synthetic gene. Assays of recombinant WAMP-1a activity showed that the peptide possessed high broad-spectrum inhibitory activity against diverse chitin-containing and chitin-free pathogens, with IC50 values in the micromolar range. The discovery of a new type of AMP active against structurally dissimilar microorganisms implies divergent modes of action and discloses the complexity of plant–microbe interactions.
- Добавил в систему: Василевский Александр Александрович