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Cy5/BHQ dye–quencher pairs in fluorogenic qPCR probes: effects of charge and hydrophobicityстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 2 сентября 2016 г.
- Авторы: Farzan Valentina M., Aparin Ilya O., Veselova Olga A., Podkolzin Alexander T., Shipulin German A., Korshun Vladimir A., Zatsepin Timofei S.
- Журнал: Analytical Methods
- Том: 8
- Номер: 29
- Год издания: 2016
- Издательство: Royal Society of Chemistry
- Местоположение издательства: United Kingdom
- Первая страница: 5826
- Последняя страница: 5831
- DOI: 10.1039/c6ay01304j
- Аннотация: For the first time we used a CuAAC click reaction for the synthesis of cyanine labeled qPCR probes. We performed the comparison of dyes and quenchers (Cy5 vs. disulfo–Cy5 and BHQ2 vs. BHQ3) in several TaqMan probes with different stability of the secondary structure. Based on the studies of thermal quenching of dyes and the increase of fluorescence during probe melting and in the course of qPCR, we suggest that the disulfo–Cy5–BHQ2 pair is a preferable one for molecular beacons due to the highest increase of fluorescence during melting. As the Cy5–BHQ3 pair gave a better Cq and a maximal relative increase of fluorescence in qPCR in comparison to others we recommend this pair for Taqman style probe design.
- Добавил в систему: Зацепин Тимофей Сергеевич